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Anandakumar, K.
- Development of UV Spectrophotometry and RP-HPLC Methods for the Estimation of Levosulpiride in Bulk and in Tablet Formulation
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Authors
Affiliations
1 Axis Clinicals Ltd, Hyderabad, Andhra Pradesh, IN
2 Dept. of Pharmaceutical Analysis, Adhiparasakthi College of Pharmacy, Melmaruvathur-603319, Tamilnadu, IN
3 Department of Pharmaceutics, Shri Vishnu College of Pharmacy, Bhimavaram, Andhra Pradesh, IN
4 Department of Pharmacology, Shri Vishnu College of Pharmacy, Bhimavaram, Andhra Pradesh, IN
1 Axis Clinicals Ltd, Hyderabad, Andhra Pradesh, IN
2 Dept. of Pharmaceutical Analysis, Adhiparasakthi College of Pharmacy, Melmaruvathur-603319, Tamilnadu, IN
3 Department of Pharmaceutics, Shri Vishnu College of Pharmacy, Bhimavaram, Andhra Pradesh, IN
4 Department of Pharmacology, Shri Vishnu College of Pharmacy, Bhimavaram, Andhra Pradesh, IN
Source
Asian Journal of Research in Chemistry, Vol 3, No 3 (2010), Pagination: 542-544Abstract
Two new simple, sensitive, rapid, accurate and precise methods, namely UV spectrophotometric and RP-HPLC methods were developed for the estimation of Levosulpiride in bulk and in tablet formulation. In UV spectrophotometric method, Levosulpiride exhibited maximum absorbance at 291.5 nm with apparent molar absorptivity of 2.6031×103 L mol-1 cm-1 in 0.1 M HCl. Beer’s law was obeyed in the concentration range of 10-50 mg/ml. In RP-HPLC method, the elution was done using a mobile phase consisting of methanol and 25 mM phosphate buffer pH 3.5 (pH adjusted with phosphoric acid, 15:85 v/v) on Shimadzu HPLC C18 (4.6×150 mm) column at a flow rate of 0.8 ml/min with UV detection at 293 nm. An external standard calibration method was employed for quantization. The elution time was 5.08 minutes. Beer’s law was found to be obeyed in the concentration range of 4-24 ×g/ml. The results of proposed methods were validated statistically and by recovery studies. The % RSD values for recovery studies were found to be less than 2% for the both methods. Hence the proposed methods were successfully used to determine the drug content in bulk and in tablet formulation.Keywords
Levosulpiride, UV Spectrophotometry, RP-HPLC, External Standard Calibration.- Estimation of Tadalafil in Bulk and in Formulation by UV-Visible Spectrophotometry
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Authors
Affiliations
1 Adhiparasakthi College of Pharmacy, Melmaruvathur-603319, Tamil Nadu, IN
1 Adhiparasakthi College of Pharmacy, Melmaruvathur-603319, Tamil Nadu, IN
Source
Asian Journal of Research in Chemistry, Vol 3, No 1 (2010), Pagination: 54-57Abstract
Two simple, precise and accurate methods were developed for the estimation of Tadalafil in bulk and in formulations. In method A, UV spectra of tadalafil in methanol and water (80:20) exhibits the λmax of 284.5 nm. Method B is based on the formation of bluish green colored complex by the reduction of ferric ions into ferrous ions in presence of potassium ferricyanide as oxidizing agent exhibits the absorption maxima at 828 nm. Beer’s law was obeyed in the concentration range of 5-30 μg/ml and 2-10 μg/ml for method A and B, respectively. The accuracy of the method was determined by recovery studies. The methods were validated as per ICH guidelines. % RSD value was less than 2%; this proved that the methods are having good recovery and reproducibility. The proposed methods are simple, rapid, precise and accurate and hence can be applied for routine quality control analysis of tadalafil in bulk and in tablet dosage forms.Keywords
Tadalafil, UV-Visible Spectrophotometry, Method Validation, Ferric Chloride, Potassium Ferricyanide.- Validated RP-HPLC Method for the Estimation of Eszopiclone in Bulk and Tablet Dosage Form
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Authors
Affiliations
1 Adhiparasakthi College of Pharmacy, Melmaruvathur-603319, Tamil Nadu, IN
1 Adhiparasakthi College of Pharmacy, Melmaruvathur-603319, Tamil Nadu, IN